FIG. 1.

(A) Comparative immunoblot with French press extracts from S. elongatus wild-type (WT) and mutant strains carrying antibiotic resistance cassettes in the chromosomal DNA region upstream of idiA. Cells were grown for 3 days under iron-sufficient conditions (+Fe growth) or iron-deficient conditions (-Fe growth). After preparation of French press extracts, samples containing 25 μg of protein were subjected to SDS-PAGE, transferred to nitrocellulose, and immunostained with an anti-IdiA antiserum (dilution, 1:20,000). (B) Location of antibiotic resistance cassettes inserted near the idiA locus. The AMC strains are mutants that carry insertions at different positions. The hash marks indicate breaks in the scale required to include several loci on the map. The numbers are positions in the EMBL database accession no. Z48754 entry. The asterisk indicates the position of the previously mapped 5′ transcript end.