FIG. 6.

Time course of idiA::luxAB gene expression in AMC824 under iron-sufficient, -depleted, and -replete conditions. Strains were grown under iron-sufficient or -deficient conditions. (A) Single time course assay with AMC824. The dashed line indicates when the regular amount of iron components in BG-11M medium (30 μM ferric ammonium citrate) was added to the iron-replete culture. Symbols: ◊, iron-sufficient conditions; □, iron-deficient conditions; ▵, iron-deficient and -replete conditions. (B) Average values (with error bars) for the key time points in three experiments like those whose results are shown in panel A. Open bars, iron-deficient growth conditions; solid bars, iron-deficient and -replete growth conditions.