FIGURE 1.

Layout of the PRRSV-2 vaccination and challenge animal trial. Four-week old weaner pigs were distributed into 10 groups—five MOCK– and five Prevacent-vaccinated groups. Pigs were vaccinated at –28 days post challenge (dpc). At day of challenge (0 dpc), each of the five groups received one of five intranasal inoculations—MOCK, NADC30, NC174, VR2332, or NADC20. At 14 dpc, pigs were sacrificed to assess lung pathology. As indicated in the timeline, blood and nasal swabs were collected throughout the study to assess viral loads as well as the humoral and T-cell immune response.