FIGURE 2.

Heterologous vaccine efficacy of Prevacent—rectal temperatures, viremia, and viral loads in nasal swabs. Rectal temperatures (A), viremia (B), and (C) viral loads in nasal swabs were determined at 0, 7, and 14 days post challenge (dpc) with MOCK (gray), or the PRRSV strains 1–4–4 (NADC30, dark blue), NC174 (red), VR2332 (green), or 1–4–2 (NADC20, light blue). The line graphs in panel (A) illustrate the means with standard deviation of rectal temperatures [°C]. Viremia (B) and viral shedding (C) were quantified by PRRSV-specific qPCR in serum and nasal swabs, respectively (genomic copy numbers/mL [log10]). The black bars represent the median values; in addition, individual data points are shown for MOCK vaccinated animals (open diamonds) and MLV vaccinated animals (filled squares). The data were analyzed using Šidák multiple comparison test 2-way ANOVA. Each vaccinated group was compared to their respective PRRSV type-2 challenge MOCK vaccinated group within each timepoint. ^****p < 0.0001, ^***p < 0.001, ^**p < 0.01, *p < 0.05.