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. 2022 Sep 23;9:1011715. doi: 10.3389/fvets.2022.1011715

Table 7.

Effects of dietary supplementation with β-glucan and/or Bacillus coagulans on intestinal digestive enzymes and histomorphometry features of tilapia fish.

Parameters Control Dietary treatments Pooled SEM p-value
CNT BG BC1 BC2 BG+BC1 BG+BC2
Digestive enzymes
Amylase (U/L) 103.7 100.5 103.9 104.1 107.5 106.7 3.77 0.807
Lipase (U/L) 82.9 85.49 86.61 93.83 91.17 82.24 3.05 0.173
Histomorphometrically characterization
VL (μm) 148.5f 158.4e 192.3d 226.9b 274.1a 201.7c 2.19 < 0.001
CD (μm) 25.76a 24.53ab 23.16ab 22.03b 21.10b 21.3b 1.02 0.040
VL/CD 5.32d 6.64c 9.60b 9.84b 11.04a 10.61a 0.15 < 0.001
VW (μm) 58.55b 59.41b 64.90a 64.26a 65.52a 59.06b 1.27 0.004
IVS (μm) 22.42a 19.71b 17.47c 16.82cd 15.25e 16.09de 0.31 < 0.001
VSA (μm2) 9123.0c 9602.6bc 7612.6c 12179abc 14723a 13993ab 3.95 0.020
GC count/mm2 18.9f 19.96e 24.23d 27.33c 31.23a 30.26b 0.30 < 0.001

VL, Villi length; CD, Crypt depth; VW, Villi width; IVS, Inter-villi space; VSA, Villi surface area; GC, Goblet cells.

One-way ANOVA non-significant for P > 0.05. The different letters within the same raw indicated to significance variation. Data were presented as the mean ± pooled standard error.