TABLE 1.
Pairwise sequence identities of halohydrin dehalogenases and SDRs
| Enzyme acronym and function | Organism | Source or reference | Identity (%)
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HheA | HHeAAD2 | HheB | HheBGP1 | HheC | HalB | 1AE1 | 2AE1 | 1AHH | 2HSD | 1AU4 | |||
| Halohydrin dehalogenases | |||||||||||||
| Group A | |||||||||||||
| HheA | Corynebacterium sp. strain N-1074 | 36 | 100 | 97.1 | 24.1 | 24.0 | 32.0 | 29.2 | 24.3 | 24.1 | 25.5 | 24.2 | 19.9 |
| HheAAD2 | Arthrobacter sp. strain AD2 | This study | 100 | 24.5 | 24.2 | 32.4 | 29.6 | 24.6 | 24.1 | 25.1 | 23.5 | 20.2 | |
| Group B | |||||||||||||
| HheB | Corynebacterium sp. strain N-1074 | 36 | 100 | 98.4 | 25.4 | 23.9 | 20.9 | 18.7 | 22.2 | 24.2 | 18.0 | ||
| HheBGP1 | Mycobacterium sp. strain GP1 | This study | 100 | 25.8 | 23.9 | 20.2 | 18.7 | 21.5 | 23.8 | 18.2 | |||
| Group C | |||||||||||||
| HheC | A. radiobacter AD1 | This study | 100 | 80.7 | 22.2 | 23.1 | 25.5 | 23.7 | 20.4 | ||||
| HalB | A. tumefaciens | —a | 100 | 17.6 | 20.6 | 22.6 | 21.7 | 20.2 | |||||
| SDR oxidoreductases | |||||||||||||
| 1AE1 (tropinone reductase I) | Datura stramonium | 8 | 100 | 61.5 | 29.7 | 27.4 | 21.9 | ||||||
| 2AE1 (propinone reductase II) | D. stramonium | 8 | 100 | 32.4 | 27.6 | 20.4 | |||||||
| 1AHH (7α-hydroxysteroid dehydrogenase) | E. coli | 29 | 100 | 32.2 | 19.7 | ||||||||
| 2HSD (20-β-hydroxysteroid dehydrogenase) | Streptomyces exfoliatus | 11 | 100 | 22.6 | |||||||||
| 1A4U (alcohol dehydrogenase) | Drosophila lebanonensis | 3 | 100 | ||||||||||
a
—, unpublished (accession no. AAD34609).