FIG. 2.

(A) Construction of plasmids containing various regions of the AP operon. DNA fragments used for subcloning are indicated by open bars below the physical map. (B) Expression profile of the AP dioxygenase from strains with different plasmids. The carbon source used as a growth substrate or an inducer is indicated in parentheses (NB, nitrobenzene; AP, 2-aminophenol; suc, succinate). 12, HS12; 124, HS124; 120, HS120. Plasmid pUP3951 was constructed by cloning a 1.0-kb SalI-SphI DNA fragment (the nbzB gene) from pNB2 (37) into pUCP22.