TABLE 2.
Enzyme activities of AP catabolic enzymes in various plasmids
| Plasmidb | Enzyme sp act (nmol/min/mg of protein)a
|
||||||
|---|---|---|---|---|---|---|---|
| NbzC (dioxygenase) | NbzD (dehydrogenase) | NbzE (deaminase) | NbzF (decarboxylase) | NbzG (hydratase) | NbzH (aldolase) | NbzI (dehydrogenase) | |
| pHS29 | 69 ± 3 | 46 ± 3 | 314 ± 25 | 2,104 ± 23 | 80 ± 4 | 171 ± 12 | 106 ± 15 |
| pHS291 | 175 ± 20 | 74 ± 5 | 164 ± 15 | 3,510 ± 29 | 113 ± 6 | — | — |
| pHS2911 | 333 ± 21 | 208 ± 16 | — | — | — | — | — |
| pHS2912 | —c | 304 ± 13 | — | — | — | — | — |
| pHS2913 | — | 262 ± 28 | — | — | 209 ± 19 | — | — |
| pHS2915 | — | — | 480 ± 27 | 7,326 ± 542 | 365 ± 32 | — | — |
| pHS2916 | — | — | 725 ± 46 | 4,534 ± 359 | — | — | — |
| pHS2920 | — | — | 613 ± 35 | — | — | — | — |
| pHS2927 | — | — | — | — | — | — | 359 ± 58 |
| pHS2928 | — | — | — | — | — | 113 ± 25 | — |
a
Enzyme assays were performed with cell extracts prepared as described in Materials and Methods and each enzyme activity is a mean value of three independent measurements.
b
All E. coli plasmids were not induced.
c
—, not detected.