TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant properties | Source or reference |
|---|---|---|
| Y. pestis | ||
| KIM8-3002 | pCD1 (Lcr+) pMT1 Pla− Smr | 21 |
| KIM8-3002.6 | KIM8-3002 ΔlcrG2 [LcrGΔ5-95]a | 21 |
| KIM8-3002.7 | KIM8-3002 ΔlcrG3 [LcrGΔ6-86]a | 7 |
| KIM8-3002.8 | KIM8-3002 ΔlcrGV2 [LcrGΔ6-95]a [LcrVΔ1-268]a | 7 |
| E. coli | ||
| DH5α | φ80dlacZΔM15 Δ(lacZYA-argF) U169 deoR recA1 endA1 hsdR17 (rK− mK+) phoA supE44 thi-1 gyrA96 relA1 | Life Technologies |
| DH5α/λpir | DH5α λpir | S. Straley |
| Novablue | recA1 endA1 hsdR17 (rK− mK+) supE44 thi-1 gyrA96 relA1 lac [F′ proA+B+lacIqZΔM15::Tn10] | Novagen |
| S. cerevisiae Y190 | MATa ura3-52 his3-200 lys2-801 ade2-101 trp1-901 leu2-3 112 gal4Δ gal80Δ cyhr2 LYS2::GAL1UAS-HIS3TATA-HIS3 URA3::GAL1UAS-GAL1TATA-lacZ | Clontech |
| Plasmids | ||
| pBAD18-Kan | araBADp cloning vector, Kmr | 10 |
| pAraG18K | pBAD18-Kan + lcrG | 22 |
| pAraV18K | pBAD18-Kan + lcrV | 22 |
| pAraGV18K | PBAD18-Kan + lcrGV | 22 |
| pJM89 | pBAD18-Kan + lcrG (A16R) | This study |
| pJM90 | pBAD18-Kan + lcrG (A16D) | This study |
| pACT2 | GAL4(768-881) AD LEU2 Apr | Clontech |
| pACTG | pACT2 + lcrG | This study |
| pJM91 | pACT2 + lcrG (A16R) | This study |
| pJM92 | pACT2 + lcrG (A16D) | This study |
| pAS2-1 | GAL4(1-147) DNA-BD TRP1 AprCYHS2 | Clontech |
| pJM17 | pAS2-1 Δcyh2 | This study |
| pASV | pAS2-1 + lcrV | This study |
| pJM15 | pASV Δcyh2 | This study |
| pLD55 | Apr Tcr suicide vector | 16 |
| pMNΔlcrGV2 | pLD55 + ΔlcrGV2 allele | This study |
| pAra-HT-V | pBAD24 + His6-LcrV, Apr | This study |
a
Amino acids deleted are indicated.