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Yeast two-hybrid analysis of LcrG and LcrV interactions
| Derivative of pACT2 | Presence of LcrVa | Colony lift assay | β-Gal activity (Miller units) |
|---|---|---|---|
| pACT-LcrG | + | + | 57.7 |
| pACT2 | + | − | 5.2 |
| pACT-LcrG | − | − | 7.3 |
| pJM91 (A16R) | + | − | 4.5 |
| pJM92 (A16D) | + | − | 4.1 |
Vector control (−) or vector with LcrV (+) supplied.
