FIGURE 1.

(A) Representative TLC showing lipid profiles of OMM and IMM lipid extracts. TLC was prepared by spotting aliquots taken from membrane lipid extracts containing a fixed amount (5 μg) of total lipid phosphorus. The long tail of the PC standards is observed at high lipid concentration. (B) Representative TLC showing the lipid profile of IMM using three different running solvents (see methods for details). (C) Representative TLC showing the lipid profile of OMM using three different running solvents. The lipid abbreviations correspond to: Chol, cholesterol; CL, cardiolipin; PE, phosphatidylethanolamine, PS, phosphatidylserine; PI, phosphatidylinositol; phosphatidylcholine; SM, sphingomyelin.