TABLE 2.
HSP83/HSPC co-chaperones from Trypanosoma brucei with their putative orthologues in T. cruzi, L. major, C. fasciculata, B. saltans, and H. sapiens.
| H. sapiens | H. sapiens | T. brucei | T. cruzi 3 | L. major | C. fasciculata | B. saltans | |||
|---|---|---|---|---|---|---|---|---|---|
| Name | Gene ID1 | Gene ID1 | Gene ID1 | Gene ID1 | Gene ID1 | Gene ID1 | Localization2 | Reference | |
| A: TPR-containing Hsp90 co-chaperones | |||||||||
| STi1/HOP | 10963 | Tb927.5.2940 | Tc_MARK_9009 | LmjF08.1110 | CFAC1_020023900 | BSAL_57725 | CYTO | Gunasekera et al. (2012); Urbaniak et al. (2012); Butter et al. (2013); Shimogawa et al. (2015); Dean et al. (2017) | |
| NUC | |||||||||
| Tbg972.5.4130 | C4B63_59g115 | CELL SURFACE (BSF, PF) | |||||||
| PP5 | 5536 | Tb927.10.13670 Tbg972.10.16800 | TcCLB.507.993.190 | LmjF.18.0150 | CFAC1_140007400 | BSAL_15705 | CYTO (BSF, PF) | Gunasekera et al. (2012); Urbaniak et al. (2012); Butter et al. (2013); Dean et al. (2017) | |
| C4B63_4g368 | |||||||||
| Cyp40 | 5481 | Tb927.9.9780 Tbg972.9.5630 | TcCLB.506,885.400 Tc_MARK_4311 | LmjF.35.4770 | CFAC1_300099000 | BSAL_06490 | CYTO | Oberholzer et al. (2011); Dean et al. (2017) | |
| C4B63_2g294 | FLAGELLAR (BSF) | ||||||||
| DnaJC7 | 7266 | Tb927.10.4900 | TcCLB.504.203.60 | LmjF.36.0500 | CFAC1_250012000 | BSAL_30720 | CYTO | Urbaniak et al. (2012); Butter et al. (2013); Dean et al. (2017) | |
| Tc_MARK_8493 | |||||||||
| Tbg972.10.5950 | C4B63_13g112 | NUC (BSF, PF) | |||||||
| FKBP5 | 2289 | Tb927.10.16100 Tbg972.10.19710 | TcCLB.511.353.10 Tc_MARK_4665 | LmjF.19.1530 | CFAC1_210025000 | BSAL_03610 BSAL_65235 | CYTO | Gunasekera et al. (2012); Urbaniak et al. (2012); Butter et al. (2013); Subota et al. (2014); Shimogawa et al. (2015); Dean et al. (2017) | |
| C4B63_157g28 C4B63_171g30 | FLAGELLAR (BSF, PF) | ||||||||
| SGT | 6449 | Tb927.6.4000 Tbg972.6.3780 | TcCLB.511.737.10 Tc_MARK_2022 | LmjF.30.2740 | CFAC1_260051600 | BSAL_66445 | CYTO | Gunasekera et al. (2012); Urbaniak et al. (2012); Butter et al. (2013); Subota et al. (2014); Shimogawa et al. (2015); Dean et al. (2017) | |
| FLAGELLAR | |||||||||
| C4B63_18g260 | CELL SURFACE (BSF, PF) | ||||||||
| TPR domain protein | 7268 | Tb927.10.11380 | TcCLB.507.709.70 | LmjF.33.0700 | CFAC1_280016100 | CYTO | Urbaniak et al. (2012); Butter et al. (2013); Dean et al. (2017) | ||
| Tbg972.10.13740 | Tc_MARK_3,620 | ||||||||
| C4B63_84g34 | |||||||||
| B: CS-containing Hsp90 co-chaperones | |||||||||
| p23 | 10728 | Tb927.9.10230 Tb927.10.2620 Tbg972.9.5930 Tbg972.10.3260 | TcCLB.509.551.70 TcCLB.506.407.60 | LmjF.35.4470 LmjF.34.0210 | CFAC1_300096200 CFAC1_290030000 | BSAL_38665 | CYTO | Dean et al. (2017) | |
| FLAGELLAR | |||||||||
| C4B63_2g235 C4B63_47g40 | NUC | ||||||||
| CHORD | 26548 | Tb927.1.3170 | TcCLB.507.837.40 | LmjF.20.1620 | CFAC1_170031900 | BSAL_00100 | CYTO (BSF, PF) | Gunasekera et al. (2012); Urbaniak et al. (2012); Butter et al. (2013); Dean et al. (2017) | |
| Tc_MARK_7,282 | |||||||||
| Tbg972.1.1930 | C4B63_93g75 | ||||||||
| Aarsd1 | 80755 | Tb927.9.6650 | TcCLB.504.883.50 | LmjF.15.0690 | CFAC1_240038900 | BSAL_93700 | CYTO | Dean et al. (2017) | |
| Tc_MARK_5844 | |||||||||
| Tbg972.9.3510 | C4B63_136g36 | ||||||||
| PIH1D1 | 55011 | Tb927.9.10490 Tbg972.9.6100 | TcCLB.506.147.150 | LmjF.35.4330 | CFAC1_300094700 | CYTO | Dean et al. (2017) | ||
| Tc_MARK_4354 | |||||||||
| C4B63_80g31 | |||||||||
| PIH1D3 | 139212 | Tb927.3.4410 Tbg972.3.4850 | TcCLB.507.257.160 | LmjF.29.1850 | CFAC1_190042200 | BSAL_07820 | CYTO (BSF, PF) | Urbaniak et al. (2012); Butter et al. (2013); Dean et al. (2017) | |
| Tc_MARK_3,141 | |||||||||
| C4B63_10g277 | BSAL_56185 | ||||||||
| Ubiquitin hydrolase | 10869 | Tb927.9.13430 Tbg972.9.8400 | TcCLB.510.749.40 | LmjF.35.2410 | CFAC1_300073100 | BSAL_27670 | CYTO | Dean et al. (2017) | |
| Tc_MARK_4527 | |||||||||
| C4B63_2g83 | |||||||||
| CS domain/TPR repeat | 161582 | Tb927.11.16050 Tbg972.11.17990 | TcCLB.509.073.90 | LmjF.32.2850 | CFAC1_190042200 | BSAL_62790 | CYTO | Dean et al. (2017) | |
| Tc_MARK_3396 | |||||||||
| C4B63_2g215 | |||||||||
| NADH-cytochrome B5 reductase | 51167 | Tb927.11.3750 Tbg972.11.4290 | TcCLB.511.047.40 | LmjF.13.1060 | CFAC1_220039400 | BSAL_93355 | CYTO | Dean et al. (2017) | |
| Tc_MARK_6965 | |||||||||
| C4B63_46g107 | |||||||||
| LRRC | 23639 | Tb927.3.4770 Tbg972.3.5330 | TcCLB.503.671.30 | LmjF.29.2210 | CFAC1_200030000 | BSAL_68670 | CYTO | Butter et al. (2013); Dean et al. (2017) | |
| Tc_MARK_3,178 | |||||||||
| C4B63_23g85 | |||||||||
| C: Non-TPR, non-CS-containing Hsp90 co-chaperones | |||||||||
| Aha1 | 10598 | Tb927.10.13710 Tbg972.10.16840 | TcCLB.507.993.150 | LmjF.18.0210 | CFAC1_140008400 | BSAL_15670 | CYTO | Gunasekera et al. (2012); Urbaniak et al. (2012); Butter et al. (2013); Dean et al. (2017) | |
| Tc_MARK_4860 | |||||||||
| C4B63_4g357 | NUC (BSF, PF) | ||||||||
| Phosphoducin | 79031 | Tb927.11.4930 | TcCLB.507.669.130 | LmjF.24.0170 | CFAC1_21006700 | BSAL_83285 | CYTO | Dean et al. (2017) | |
| Tc_MARK_6540 | |||||||||
| Tbg972.11.5550 | C4B63_28g45 | ||||||||
The Gene IDs for the T b. brucei (Tb refers to Tbb), T b. gambiense, T. cruzi, C. fasciculata, B. saltans, and L. major HSP83/HSPC co-chaperones were retrieved from the TriTrypDB database (http://tritrypdb.org/tritrypdb/; Aslett et al., 2010). The Gene IDs for the members of the H. sapiens HSP90/HSPC co-chaperones were retrieved from NCBI (https://www.ncbi.nlm.nih.gov/).
The Gene IDs, for the orthologues, identified by reciprocal BLASTP analysis, of three strains of T. cruzi are listed. T. cruzi CL, Brener Esmeraldo-like (TcCLB), T. cruzi Dm28c 2018 (C4B63), and T. cruzi marinkelli strain B7 (Tc_MARK).
Subcellular localizations for the T. brucei HSP83/HSPC co-chaperone proteins were acquired from using the TrypTag database (http://tryptag.org/; Dean et al., 2017) and/or determined using various proteomic datasets listed in the materials and methods.
CYT-Cytosol; MITO- mitochondrion; NUC- nucleus; ER- endoplasmic reticulum; GYLCO-glycosomes; FLAGELLAR- flagellar; CELL SURFACE- cell surface.