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. 2022 Sep 23;13:1013304. doi: 10.3389/fpls.2022.1013304

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Copyright © 2022 Sakuraba

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression profiles of seven genes encoding chlorophyll catabolic enzymes during N deficiency. Expression levels of seven chlorophyll catabolic enzyme-encoding genes NYC1, NOL, HCAR, SGR1, PPH, PAO, and RCCR, and a high-affinity nitrate transporter-encoding gene NRT2.5 (positive control) in the shoots of Arabidopsis Col-0 (wild type) seedlings are shown. Plants were grown in plates containing half-strength Murashige and Skoog (1/2 MS)-agar medium for 7 days and then under N-deficient conditions (0.3 mM N) for the indicated time periods. Transcript levels of each gene were normalized against the transcript levels of ACTIN2 (ACT2) and then against the value obtained from samples at time zero. Data represent mean ± standard deviation (SD) of four biological replicates.