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. 2022 Sep 19:10.1002/jmv.28135. Online ahead of print. doi: 10.1002/jmv.28135

Figure 1.

Figure 1

Schematics of the L1RP EGFP and JM111 constructs. (A) The L1RP EGFP construct included both the L1 5′‐UTR and the CMV promoter. The JM111 construct included two point mutations (R261A/R262A) in ORF1, rendering it transposition‐incompetent. (B) Description of the retrotransposition assay. EGFP can only be identified when the intron is removed after RNA splicing and when L1 is successfully transposed into the genome. (C) Genome organization of SARS‐CoV‐2. CMV, cytomegalovirus; EGFP, enhanced green fluorescent protein; L1RP, a full‐length L1 element that has inserted into the retinitis pigmentosa‐2 (RP) gene; ORF, open reading frame; SARS‐CoV‐2, severe acute respiratory syndrome coronavirus 2; UTR, untranslated region.