FIG. 2.

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of ORF2 purification steps. The E. faecalis UDP-MurNAc-pentapeptide:l-alanine ligase was purified from E. coli JM83/pDA29(orf2) crude extract (lane E) by anion exchange chromatography (lane 1) and by ammonium sulfate precipitation (lane 2), followed by hydrophobic-interaction (lane 3), affinity (lane 4), and exclusion (lane 5) chromatographies. Lanes 1 to 5 refer to fractions 1 to 5, respectively, as described in Materials and Methods.