TABLE 1.
Synthesis of UDP-MurNAc-hexapeptide by W. viridescens and E. coli extractsa
| Source of:
|
Hexapeptide production | |
|---|---|---|
| UDP-MurNAc-pentapeptide:l-alanine ligase | Alanyl-tRNA synthetase | |
| W. viridescens fraction I | W. viridescens fraction I | + |
| W. viridescens fraction III | W. viridescens fraction IV | + |
| W. viridescens fraction III | None | − |
| None | W. viridescens fraction IV | − |
| W. viridescens fraction III | E. coli JM83/pDA26(alaSEfa) crude extract | + |
| E. coli JM83/pDA28(orf1) crude extract | W. viridescens fraction IV | − |
| E. coli JM83/pDA29(orf2) crude extract | W. viridescens fraction IV | + |
a
Addition of l-[14C]alanine to UDP-MurNAc-pentapeptide required tRNA (W. viridescens fraction II) and was suppressed if RNase was added to the reaction mixture. l-[14C]alanine and [14C]UDP-MurNAc-hexapeptide were separated by paper chromatography.