Figure 1.

Illustration of 20 single‐point spike mutation pseudovirus and Phylogenetic analysis of ACE2 orthologs. (A) Schematic of 20 SARS‐CoV‐2 spike mutations. (B) Western blot detection of spike proteins of 20 single‐point mutation pseudovirus using an antibody against the HA tag conjugated to the viral spike proteins. HIV‐1 p24, a protein of the carrier pseudovirus, was detected as the loading control. (C) The sequences of 20 ACE2 proteins were analyzed and the phylogenetic tree was built. The residues of human ACE2 at the SARS‐CoV‐2 RBD/ACE2 interface are shown. ACE2, angiotensin‐converting enzyme 2;  RBD, receptor‐binding domain.