TABLE 2.
Distribution of CODH I and CODH II on ultrathin sections of CO-grown C. hydrogenoformansa
| Source (phase) of bacterial cells | CODH I in:
|
CODH II in:
|
||
|---|---|---|---|---|
| Cytoplasmic membrane, total count/cellb (%c) | Cytoplasm, total count/cellb (%c) | Cytoplasmic membrane, total count/cellb (%c) | Cytoplasm, total count/cellb (%c) | |
| Middle of exponential growth | 6.8 ± 1.2 (99.3 ± 2.9) | 0.1 ± 0.3 (0.7 ± 2.9) | 4.1 ± 1.3 (93.4 ± 9.4) | 0.4 ± 0.5 (6.6 ± 9.4) |
| End of exponential growth | 8.5 ± 1.9 (98.3 ± 3.6) | 0.2 ± 0.4 (1.7 ± 3.6) | 6.1 ± 1.4 (92.4 ± 7.6) | 0.5 ± 0.5 (7.6 ± 7.6) |
| Stationary growth | 9.0 ± 1.7 (98.8 ± 3.3) | 0.1 ± 0.3 (1.2 ± 3.3) | 5.0 ± 1.9 (94.2 ± 8.3) | 0.4 ± 0.5 (5.8 ± 8.3) |
a
Gold particles found in attachment to the cytoplasmic membrane of C. hydrogenoformans or present in the cytoplasm after postembedding labeling with the immunogold method were counted; for details see Materials and Methods.
b
Numbers refer to mean values ± standard deviations of gold particles on equally sized cross-sections of about 30 cells on different grids.
c
Percentage of gold particles in the indicated cell fraction relative to the total count of gold particles per cell, which was set 100%.