TABLE 3.
Effect of CODH I and CODH II on the CO-dependent formation of H2 by cytoplasmic membranes of CO-grown C. hydrogenoformans
| Assay conditionsa | H2 evolutionb (U) |
|---|---|
| Cytoplasmic membranes plus CODH I plus protein B | 30.1 |
| Cytoplasmic membranes plus CODH I | 8.1 |
| Cytoplasmic membranes plus CODH II plus protein B | 8.8 |
| Cytoplasmic membranes plus CODH II | 8.8 |
| Cytoplasmic membranes | 8.1 |
| CODH I | 0 |
| CODH II | 0.7 |
| B protein | 0 |
| CODH I plus B protein | 0 |
| CODH II plus B protein | 0.8 |
a
Assays contained 5 mg of CODH I or CODH II, 5 mg of protein B, and 1 mg of cytoplasmic membranes as indicated. Cytoplasmic membranes were prepared as detailed in Materials and Methods and washed in buffer A by ultracentrifugation.
b
H2 formation was measured under an atmosphere of 100% CO.