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. 2022 Sep 23;16:988732. doi: 10.3389/fncel.2022.988732

FIGURE 3.

FIGURE 3

The number of GABA+ cells was increased by VPA treatment. (A) Representative images showing that the control (A1–3) and VPA-treated (A4–6) brains were immunostained with an anti-GABA antibody at stage 48. Arrows indicate the GABA+ cells. Scale bar = 50 μm. (B) The data shows that the GABA+ cells were significantly increased by VPA treatment. GABA: Ctrl, 690.2 ± 13.4, N = 5; VPA, 909.7 ± 28.3, N = 6). N represents the number of tadpoles. (C,D) Western blot of H3K9ac and summary of data showing that the relative intensity of H3K9 to GAPDH was significantly increased by VPA treatment. N = 12. ***p < 0.001. N represents the number of independent experiments.