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. 2022 Aug 21:10.1002/ddr.21982. Online ahead of print. doi: 10.1002/ddr.21982

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© 2022 Wiley Periodicals LLC.

This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.

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Celastrol does not affect cell viability. Alamar Blue assay was used to measure the potential cytotoxic effect of celastrol. The monkey cell line (a) Vero CCL‐81 and the human cell lines (b) Caco‐2 and (c) Calu‐3 were treated with celastrol at 250 and 1000 nM, for 48 h. DMSO solutions at 0.05% and 5.0% were used as the negative and positive controls of cell death, respectively. Statistical analysis was performed using ANOVA followed by Tukey's posttest to compare treatment with celastrol and 5.0% DMSO. ****p < .0001. ANOVA, analysis of variance.