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. 2022 Sep 23;13:981825. doi: 10.3389/fimmu.2022.981825

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Copyright © 2022 Lu, Baertsch, Hickey, Goltsev, Rech, Mani, Forgó, Kong, Jiang, Nolan and Rosenthal

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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3D image deconvolution improves image contrast in CODEX data. (A) Representative raw and deconvolved images of a cervical lymph node, and plot of normalized fluorescence intensity versus lateral position of a selected cell corresponding to the raw and deconvolved images. (B) Quantification of the FWHM for signals cross ten randomly selected cells. (C) Representative raw and deconvolved images of cytokeratin 19-stained pancreatic ducts and corresponding predicted whole-cell segmentation mask. Wilcoxon matched-pairs signed rank test was used in (B). **P < 0.01.