FIG. 2.

Single-label FISH experiments showing the subcellular localizations of three chromosomal loci. Cy3-labeled oriC and TIR probes and the digoxigenin-labeled LAdj probe were used to detect the subcellular localizations of the replication origin region (A to D), the chromosome ends (E), and a nonrepeated region adjacent to the left TIR (F) in S. coelicolor substrate mycelia. (A) Internal portion of the mycelium. (B) Branch region of the mycelium. Green arrows point to branch regions. (C and D) Mycelial tips. White arrows point to twin oriC spots. Scale bar = 1 μm.