TABLE 3.
Over‐representation test for classes of genes with changes across three groups with direction of change in expression
| Molecular function | Mus Mus genes | Enriched | Expected | Fold enrichment | Significance | A vs B | A vs C | A vs D | Genes |
|---|---|---|---|---|---|---|---|---|---|
| G protein‐coupled receptor activity (GO:0004930) | 405 | 3 | 0.56 | 5.32 | 0.02 | Down | Up | Up | Aplnr; Sstr2; Adgrl4 |
| Neuropeptide binding (GO:0042923) | 21 | 1 | 0.03 | 34.2 | 0.03 | Down | Up | Up | Sstr2 |
| Peptide binding (GO:0042277) | 179 | 2 | 0.25 | 8.02 | 0.03 | Down | Up | Up | Sstr2; Aplnr |
| Amide binding (GO:0033218) | 194 | 2 | 0.27 | 7.4 | 0.03 | Down | Up | Aplnr; Sstr2 | |
| Cyclin‐dependent protein serine/threonine kinase regulator activity (GO:0016538) | 35 | 1 | 0.05 | 20.52 | 0.05 | Down | Up | Ccnb2 | |
| Molecular transducer activity (GO:0060089) | 1349 | 5 | 1.88 | 2.66 | 0.04 | Down | Up | Aplnr; Pdgfrb; Ccnb2; Sstr2; Adgrl4 | |
| Rac GTPase binding (GO:0048365) | 26 | 1 | 0.04 | 27.62 | 0.04 | Down | Up | Nck1l | |
| Acetylcholine binding (GO:0042166) | 28 | 2 | 0.12 | 17.1 | <0.01 | Up | Down | Down | Lypd1; Chrm2 |
| Acetylcholine receptor activity (GO:0015464) | 28 | 2 | 0.12 | 17.1 | 0.007 | Up | Down | Down | Lypd1; Chrm2 |
| Actin filament binding (GO:0051015) | 84 | 2 | 0.35 | 5.7 | 0.05 | Up | Down | Down | Myom1; Tpm2 |
| Cation transmembrane transporter activity (GO:0008324) | 424 | 7 | 1.77 | 3.95 | <0.01 | Up | Down | Down | Slc12a5; Slc5a3; Atp7b; Slc24a4; Kcnh4; Atp2a1; Pkd1l2 |
| Hormone binding (GO:0042562) | 52 | 2 | 0.22 | 9.21 | 0.02 | Up | Down | Down | Lypd1; Chrm2 |
| Inorganic cation transmembrane transporter activity (GO:0022890) | 395 | 7 | 1.65 | 4.24 | <0.01 | Up | Down | Down | Slc12a5; Slc5a3; Atp7b; Slc24a4; Kcnh4; Atp2a1; Pkd1l2 |
| Inorganic molecular entity transmembrane transporter activity (GO:0015318) | 550 | 8 | 2.3 | 3.48 | <0.01 | Up | Down | Down | Slc12a5; Slc5a3; Atp7b; Slc24a4; Kcnh4; Slc7a5; Atp2a1; Pkd1l2 |
| Ion transmembrane transporter activity (GO:0015075) | 581 | 8 | 2.43 | 3.3 | <0.01 | Up | Down | Down | Slc12a5; Slc5a3; Atp7b; Slc24a4; Kcnh4; Slc7a5; Atp2a1; Pkd1l2 |
| Metal ion transmembrane transporter activity (GO:0046873) | 310 | 7 | 1.29 | 5.41 | <0.001 | Up | Down | Down | Slc12a5; Slc5a3; Atp7b; Slc24a4; Kcnh4; Atp2a1; Pkd1l2 |
| Monovalent inorganic cation transmembrane transporter activity (GO:0015077) | 261 | 5 | 1.09 | 4.59 | <0.01 | Up | Down | Down | Scl12a5; Slc5a3; Kcnh4; Atp2a1 |
| Postsynaptic neurotransmitter receptor activity (GO:0098960) | 49 | 2 | 0.2 | 9.77 | 0.02 | Up | Down | Down | Lypd1; Chrm2 |
| Transmembrane transporter activity (GO:0022857) | 701 | 8 | 2.93 | 2.73 | <0.01 | Up | Down | Down | Slc12a5; Slc5a3; Atp7b; Slc24a4; Kcnh4; Slc7a5; Atp2a1; Pkd1l2 |
| Ammonium ion binding (GO:0070405) | 80 | 2 | 0.33 | 5.99 | 0.05 | Up | Down | Lypd1; Chrm2 | |
| Calcium ion transmembrane transporter activity (GO:0015085) | 84 | 3 | 0.35 | 8.55 | <0.01 | Up | Down | Slc24a4; Atp2a1; Pkd1l2 | |
| Potassium ion transmembrane transporter activity (GO:0015079) | 139 | 3 | 0.58 | 5.17 | 0.02 | Up | Down | Slc12a5; Kcnh4 | |
| Transporter activity (GO:0005215) | 782 | 9 | 3.27 | 2.76 | <0.01 | Up | Down | Slc12a5; Kcnh4; Pkd1l2 | |
| Extracellular matrix structural constituent (GO:0005201) | 11 | 1 | 0.05 | 21.76 | 0.05 | Up | Up | Fbn1 | |
| Oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen (GO:0016702) | 10 | 1 | 0.04 | 23.94 | 0.04 | Up | Up | Ido1 | |
| DNA‐binding transcription factor activity (GO:0003700) | 618 | 2 | 0.31 | 6.55 | 0.04 | Down | Down | Hif3a; Egr3 |
Note: Genes with differential expression were assessed for over‐enrichment using PANTHER version 15.0, GO‐slim molecular function. Binomial test with Bonferroni correction was applied to compare the list of differentially expressed genes with that expected. Data are shown from classes identified in more than one comparison.