FIG. 1.

Expression of native Pfu-sHSP. Protein expression is shown by SDS–15% PAGE and Western blot analysis in panels A and B, respectively. (A and B) Lanes 1, culture growing at 95°C; lanes 2, 3, and 4, P. furiosus cultures after heat shock treatment at 105°C for 30, 60, and 120 min, respectively. The vertical bars on the left show the section of the gel represented in the Western blot analysis. Northern blot analyses of Pfu-sHSP gene mRNA expression and P. furiosus GDH gene mRNA (control) expression are shown in panels C and D, respectively. (C and D) Lanes 1, autoradiogram of a blot of mRNA from non-heat-shocked cells; lanes 2, corresponding mRNA signals from heat-shocked cells.