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. 2022 Sep 23;9:1010859. doi: 10.3389/fnut.2022.1010859

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Copyright © 2022 Gong, Lu, Liu, Xi, Zhang, Liu, Jin, Yang, Zhu, Xie and Han.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dietary BBR suppressed the HCD-induced apoptosis in the intestine of largemouth bass. (A) TUNEL (green) staining images of intestinal sections (arrows indicate TUNEL signal, bar = 50 μm). (B) Apoptosis rate in intestinal sections. (C) Expression level of proapoptotic genes. (D) Intestinal Caspase 3 and Caspase 9 level. (E) Transmission electron microscope images of intestinal mitochondria (arrows indicate mitochondria, bar = 1 μm). (F) Expression level of anti-apoptotic genes. Data are represented as means ± SEM (n = 6). *, **, ***, ****Different from each other: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, NS means no significant difference (One-way ANOVA, Duncan’s post hoc test).