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. 2022 Jul 2;162(3):245–261. doi: 10.1111/jnc.15656

FIGURE 2.

FIGURE 2

DJ‐1 can only repair reversible adducts of MG and guanine in vitro. (a) the addition of DJ‐1 at the same time as MG and dG (+DJ‐1 beginning; blue) reduces levels of both reversible cMG‐dG and irreversible MG‐CEdG adducts, while the addition of DJ‐1 after 1 h of preincubation of MG and dG (+DJ‐1; white) results in an accumulation of irreversible MG‐CEdG and cannot restore unmodified dG. (b) the C106S mutant (cyan), which removes the catalytic thiolate nucleophile, is inactive and cannot prevent dG modification. Only catalytically active DJ‐1 prevents the formation of dG adducts when added at the same time as MG. (c) the addition of DJ‐1 results in no change in the HPLC retention time of purified CEdG, an irreversible adduct. In all panels, each measurement is shown as a circle with a standard error of the mean shown in error bars.