TABLE 2.

DJ‐1 kinetic parameters with MG substrate from kinetic simulation

Rate constant	Best‐fit value	a Lower boundary	a Upper boundary
k 1 (M−1 s−1)	360	123.83	11466.67
k −1 (s−1)	7.82 × 10−2	2.62 × 10−12	4.13
k 2 (k cat ) (s−1)	4.02 × 10−2	3.82 × 10−2	4.27 × 10−2
b Calculated K M (M)	3.29 × 10−4	c 2.76 × 10−4	c 3.63 × 10−4
k 3 (M−1 s−1)	3.25	1.98	104.17
k −3 (s−1)	6.5 × 10−3	d ND	d ND

^a Lower and upper boundaries were determined by a published algorithm that varies the rate constants to find values that fall within a 0.83 relative error to the best‐fit (82).

^b The K _M was calculated using the definition $\left(K_m=\frac{k_{-1}+k_2}{k_1}\right)$.

^c Lower and upper boundaries are not determined directly in KinTek global software for steady‐state constants therefore they were calculated from the collection of rate constants that fit the data within a 0.83 relative error from the best‐fit.

^d KinTek global explorer software only estimates one rate constant when the ratio is being constrained by the equilibrium constant (500 M⁻¹).