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. 2022 Mar 29;98(5):1157–1166. doi: 10.1111/php.13615

Figure 5.

Figure 5

NPS‐2143 inhibits UV‐induced DNA damage and skin edema in mice following acute UV irradiation. Mice were exposed to 3 MED of ssUV and immediately treated on the UV‐irradiated dorsum with vehicle, 1,25(OH)2D3 or NPS‐2143 as indicated. (a) CPD or (c) 8‐OHdG by immunohistochemistry and image analysis in female SKH:hr1 mice, at 3 h post‐UV exposure, three mice per group, with similar results obtained in a second set of mice. ****P < 0.0001, ***P < 0.001, *P < 0.05, compared with UV + vehicle. n.s. (with bar) not significantly different from one another. ####, P < 0.0001 significantly different from UV + vehicle. Photomicrographs of CPD (b) or 8‐OHdG (d) in mouse skin. Black arrows point to the dark brown staining in nuclei indicating the presence of CPD or 8‐OHdG. Scale bar = 100 μm. (e) Skin edema was measured as skinfold thickness on the 4th day after UVR exposure. Data were expressed as mean change in dorsal skinfold thickness + SEM compared with non‐irradiated skinfold thickness. **P < 0.01 and *P < 0.05 when compared with UV + vehicle group. n = 5 mice per group.