FIG. 2.

Regulation by glucose of KHT1 and KHT2 promoter activity. Transformants of K. lactis strain JA6/DL4R (KHT1 KHT2 lac4) carrying single chromosomal insertions of KHT1-′lacZ and KHT2-′lacZ gene fusions, respectively, were grown to the exponential phase in YNB medium with 3% glycerol, collected by centrifugation, and resuspended at time 0 (t₀) in the same glycerol medium containing different amounts of glucose as additional carbon sources (no glucose ♦, 0.1% glucose ▴ 2% glucose ▪, and 5% glucose ●). During batch cultivation at 30°C, samples were taken at the indicated time points for determination of lacZ activity (solid symbols) and cell density (open symbols with dotted lines) OD600, optical density at 600 nm.