TABLE 1.
Yeast strains used in this study
| Strain | Relevant characteristics | Parent strain(s) | Source or reference |
|---|---|---|---|
| K. lactis | |||
| JA6 | α ade1-600 adeT-600 trp1-11 ura3-12 | SD11.U2 × W600B | 9 |
| JA6/DL4R | lac4::ura3 | JA6/DL4 (lac4::URA3) fluoroorotic acid-resistant derivative | 17 |
| JA6/CM57 | Chromosomal integration of KHT1-′lacZ fusion | JA6 (integration of pBM3157) | This work |
| JA6/CM58 | Chromosomal integration of KHT2-′lacZ fusion | JA6 (integration of pBM3158) | This work |
| JA6/DT12R | kht1::ura3::kht2 | JA6/DT12 (kht1::URA3::kht2) fluoroorotic acid resistant derivative | 38 |
| DT12R/57 | Chromosomal insertion of KHT1-′lacZ fusion | JA6/DT12R integration of pBM3157 | This work |
| DT12R/58 | Chromosomal insertion of KHT2-′lacZ fusion | JA6/DT12R integration of pBM3158 | This work |
| ST105 | RAG1 | AWJ-8c × MG1/2 | 38 |
| ST106 | rag1::URA3 | ST105 | 38 |
| S. cerevisiae EBY.VW4000 | Δhxt1-17 gal2 stl1 agt1 mph2-3 | CEN.PK2-1C | 40 |