TABLE 3.
Influence of hexose transporters on glucose induction and repression
| Genetic background | Gene on plasmid | Glucose induction at glucose concna:
|
|||||
|---|---|---|---|---|---|---|---|
|
KHT1′-lacZb
|
KHT2′-lacZb
|
LAC4b,e (2%) | PDCce (2%) | ||||
| 0.1%d | 2%d | 0.1%d | 2%d | ||||
| Wild-type | Vector | 2.7 | 5.1 | 2.1 | 0.4 | 0.2 | 5.2 |
| kht1 kht2 | Vector | 3.3 | 3.3 | 4.8 | 5.4 | 0.9 | 1.0 |
| kht1 kht2 | KHT1 KHT2 | NDf | 6.2 | ND | 1.0 | ND | ND |
| kht1 kht2 | RAG1 | ND | 4.6 | ND | 1.7 | 0.4 | 3.4 |
| kht1 kht2 | ScHXT1 | ND | 4.3 | ND | 3.3 | 0.6 | 1.0 |
| kht1 kht2 | GAL2 | ND | ND | ND | ND | 0.3 | 5.8 |
a
Fold- induction relative to glycerol (3%) cultures, mean of at least three independent experiments, deviations <15%.
b
β-galactosidase activity.
c
Pyruvate decarboxylase activity.
d
Glucose (0.1 or 2%) was added to 3% glycerol medium.
e
In gal80 mutant background.
f
ND, not determined.