Table 2.

Limit of detection for two methylated markers in ColoDefense2.0

SDC2			SEPT9			ACTB
CONC (pg/reaction)	PR (%)	Mean Ct	CONC (pg/reaction)	PR (%)	Mean Ct	CONC (pg/reaction)	PR rate (%)	Mean Ct
0	0	–	0	0	–	20,000	100	25.6 ± 0.1
50	100	35.8 ± 0.4	–	–	–	5000	100	27.3 ± 0.1
25	100	37.6 ± 0.9	–	–	–	2500	100	28.9 ± 0.1
12.5	75	38.6 ± 1.2	–	–	–	1250	100	29.8 ± 0.2
–	–	–	200	100	34.7 ± 0.2	20,000	100	25.7 ± 0.1
–	–	–	100	100	36.2 ± 0.5	10,000	100	26.8 ± 0.2
–	–	–	50	75	37.5 ± 0.8	5000	100	27.9 ± 0.2

CONC concentration, PR positive rate