TABLE 1.
Details of the contrast enhancing and x‐ray scanning parameters tested per specimen
| Specimen code | Part | Staining | Staining | Diffusion | Scan medium | Current (mA) | Exposure times (s) | Optical magnification | Pixel size(μm) | Voltage (kV) | Binning | Scan time |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AD_01 | Brain | PTA | 7d | CV | EtOH | 88 | 4 | 4 | 1.69 | 80 | Bin 1 | 4h49m |
| AD_01 | Ceph | PTA | 7d | CV | EtOH | 87 | 1 | 0.4 | 6.6 | 80 | Bin 2 | 1h34m |
| AD_02 | Ceph | PTA | 1d | PD | EtOH | 74 | 15 | 0.4 | 7.57 | 40 | Bin 2 | 7h47m |
| AD_02 | Ceph | PTA | 5d | PD | EtOH | 80 | 6 | 0.4 | 6.55 | 50 | Bin 2 | 3h48m |
| AD_02 | Ceph | PTA | 10d | PD | EtOH | 80 | 10 | 0.4 | ee | 50 | Bin 2 | 5h36m |
| AD_02 | Ceph | PTA | 25d | PD | EtOH | 79 | 8 | 0.4 | 8.26 | 50 | Bin 2 | 4h59m |
| AD_02 a | Ceph | PTA | 25d | PD | EtOH | 86 | 6.5 | 0.4 | 4.13 | 70 | Bin 1 | 8h19m |
| AD_02 | Eyes | PTA | 25d | PD | EtOH | 88 | 13 | 20 | 0.85 | 80 | Bin 2 | 6h53m |
| AD_02 a | Brain | PTA | 25d | PD | EtOH | 88 | 8 | 4 | 1.27 | 80 | Bin 1 | 9h27m |
| AD_03 | Ceph | PTA | 4d | IV | EtOH | 80 | 11 | 0.4 | 6.99 | 50 | Bin 2 | 5h57m |
| AD_03 | Ceph | PTA | 10d | IV | EtOH | 81 | 11 | 0.4 | 6.94 | 50 | Bin 2 | 6h3m |
| AD_03 | Brain | PTA | 10d | IV | EtOH | 8 | 4 | 2.53 | 80 | Bin 1 | 9h27m | |
| AD_04 | Ceph | PTA | 1d | CV | EtOH | 88 | 5 | 0.4 | 3.55 | 80 | Bin 2 | 3h35m |
| AD_04 | Ceph | PTA | 5d | CV | EtOH | 87 | 1 | 0.4 | 8.02 | 80 | Bin2 | 1h31m |
| AD_04 | Brain | PTA | 5d | CV | EtOH | 88 | 8 | 4 | 1.69 | 80 | Bin 1 | 9h50m |
| AD_05 | Ceph | PTA | 2d | CV | EtOH | 8 | 0.4 | 6 | 80 | Bin 2 | 4h32m | |
| AD_05 | Brain | PTA | 2d | CV | EtOH | 6 | 4 | 2.53 | 80 | Bin 1 | 7h38m | |
| AD_06 | Ceph | PTA | 5d | CV | EtOH | 87 | 1 | 0.4 | 8.02 | 80 | Bin2 | 1h31m |
| AD_06 | Brain | PTA | 5d | CV | EtOH | 87 | 7 | 4 | 1.69 | 80 | Bin 1 | 8h34m |
| AD_07 | Ceph | IOD | 1d | PD | EtOH | 88 | 0.7 | 0.4 | 8.02 | 80 | Bin 2 | 1h19m |
| AD_07 | Brain | IOD | 1d | PD | EtOH | 88 | 2.5 | 4 | 1.5 | 80 | Bin 1 | 4h27m |
| AD_07 | Brain | IOD/CPD | Dried | – | Air | 87 | 6 | 4 | 1.41 | 80 | Bin 1 | 7h38m |
| AD_07 | Ceph | IOD/CPD | Dried | – | Air | 88 | 4 | 4 | 5.77 | 80 | Bin 2 | 2h33m |
| AD_08 | Ceph | IOD | 1d | PD | EtOH | 74 | 8 | 0.4 | 6.55 | 40 | Bin 2 | 4h32m |
| AD_08 | Ceph | IOD | 1d | PD | EtOH | 80 | 12 | 4 | 5.4 | 50 | Bin 2 | 6h22 |
| AD_08 | Brain | IOD/CPD | Dried | – | Air | 90 | 3 | 4 | 1.5 | 80 | Bin 1 | 4h54m |
| AD_08 | Ceph | IOD/CPD | Dried | – | Air | 88 | 2 | 4 | 5.4 | 80 | Bin 2 | 1h38m |
| AD_09 | Ceph | IOD | 2d | PD | EtOH | 88 | 0.6 | 0.4 | 8.02 | 80 | Bin 2 | 1h16m |
| AD_09 | Brain | IOD | 2d | PD | EtOH | 87 | 2.5 | 4 | 1.6 | 80 | Bin 1 | 4h27m |
| AD_09 | Brain | IOD/CPD | Dried | – | Air | 75 | 5 | 4 | 1.99 | 80 | Bin 1 | 8h6m |
| AD_09 | Ceph | IOD/CPD | Dried | – | Air | 74 | 1.5 | 0.4 | 8.31 | 80 | Bin 2 | 1h10m |
| AD_10 | Ceph | IOD | 4d | PD | EtOH | 88 | 1 | 0.4 | 8.22 | 80 | Bin 2 | 1h27m |
| AD_10 | Brain | IOD | 4d | PD | EtOH | 87 | 2 | 4 | 1.93 | 80 | Bin 1 | 4h27m |
| AD_10 | Brain | IOD/CPD | Dried | – | Air | 87 | 3 | 4 | 1.69 | 80 | Bin 1 | 4h51m |
| AD_10 | Ceph | IOD/CPD | Dried | – | Air | 88 | 6 | 4 | 5.94 | 80 | Bin 2 | 3h29m |
| AD_11 | Ceph | IOD | 8d | PD | EtOH | 88 | 1 | 0.4 | 8.22 | 80 | Bin 2 | 1h27m |
| AD_11 | Brain | IOD | 8d | PD | EtOH | 88 | 2 | 4 | 1.93 | 80 | Bin 1 | 4h11m |
| AD_11 | Brain | IOD/CPD | Dried | – | Air | 75 | 4 | 4 | 1.93 | 80 | Bin 1 | 5h48m |
| AD_11 | Ceph | IOD/CPD | Dried | – | Air | 75 | 1 | 0.4 | 6.81 | 80 | Bin 2 | 1h10m |
| AD_12 | Ceph | IOD | 2d | PD | EtOH | 87 | 0.7 | 0.4 | 8.02 | 80 | Bin 2 | 1h31m |
| AD_12 | Brain | IOD | 2d | PD | EtOH | 88 | 2 | 4 | 1.93 | 80 | Bin 1 | 4h11m |
| AD_12 | Brain | IOD/CPD | Dried | – | Air | 71 | 6.5 | 4 | 2.27 | 70 | Bin 1 | 8h17m |
| AD_12 | Ceph | IOD/CPD | Dried | – | Air | 72 | 1 | 0.4 | 7.37 | 70 | Bin 2 | 1h40m |
| AD_25 | Brain | PTA | 25d | PD | EtOH | 87 | 4 | 4 | 2.05 | 80 | Bin 1 | 5h50m |
| AD_25 | Ceph | PTA | 25d | PD | EtOH | 88 | 1 | 0.4 | 8.04 | 80 | Bin 2 | 1h11m |
| AD_26 | Brain | PTA | 25d | PD | EtOH | 88 | 4 | 4 | 2.05 | 80 | Bin 1 | 5h50m |
| AD_26 | Ceph | PTA | 25d | PD | EtOH | 87 | 1 | 0.4 | 8.04 | 80 | Bin 2 | 1h11m |
Scanning parameters used. Diffusion column indicates how the staining process was done PD, passive diffusion; CV, continuous vacuum; IV, intermittent vacuum. Some samples were tested more than once under different parameters and staining times. Samples AD_07–AD_12 were scanned before and after CPD. Specimen codes used here correspond to the ones indicated in Table 2, Figures 1, 2, 3, 4, 5, 6, and Supplementary Material.
a
Indicates our preferred parameters.