TABLE 1.

Inclusion and exclusion criteria

Inclusion	Exclusion
>18 years old	Smokers
	Pregnant women
	Teeth with periodontal pockets>4 mm/endodontic—periodontal lesion
Root canal retreatment cases	Patients with chronic inflammatory condition (Asthma, Inflammatory bowel diseases, Irritable bowel syndrome, Chronic peptic ulcer, Rheumatoid arthritis, Ulcerative colitis, Liver diseases, Crohn's disease, Sinusitis, Active hepatitis, Autoimmune diseases, Tuberculosis, Renal diseases, or Cancer).
Teeth requiring periapical surgery	Patients on medication altering bone metabolism
	Unrestorable teeth
	Antibiotics in last 3 months
	Surgical procedure in last 6 months