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. 2022 Sep 2;600(18):4187–4205. doi: 10.1113/JP283021

Figure 2. Optogenetic activation of RVM projections stimulate GABA/glycine IPSCs.

Figure 2

A, pairs of light‐stimulated currents evoked in SDH target neurons voltage clamped at −50 mV. Inward currents were blocked by NBQX and d‐APV (middle). Outward currents were blocked by strychnine and SR95531 (bottom) indicating optically evoked currents are inhibitory postsynaptic currents (oIPSCs). B, bar graph of antagonist effects on the peak amplitude of the first light‐stimulated current of n = 10 cells from eight rats (P > 0.0001 (Norm vs. Stry+SR) and 0.011 (NBQX+APV vs. Stry+SR); Friedman test with Dunn‐Bonferroni post hoc test). C, oIPSCs were suppressed by the presence of the voltage‐gated sodium channel blocke tetrodotoxin (TTX, 0.5–1 μM, n = 3) and this effect was prevented by adding potassium channel blocker 4‐aminopyridine (4‐AP, 100 μM, n = 3; Petreanu et al., 2009). D, quantification of C.