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Coomassie-stained SDS-PAGE gel containing the following samples: whole-cell lysate of E. coli BL21(DE3)/pET-CRP prior to (lane 1) and 3 h after (lane 2) IPTG induction, protein standards (lane 3), and purified CRP protein (lanes 4 and 5) after affinity chromatography on cAMP agarose.
