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. 2022 Sep 23;13:996746. doi: 10.3389/fimmu.2022.996746

Figure 2.

Figure 2

Siglec-7+ CD8+ T cells display a higher oxidative phosphorylation capacity at steady state. (A, B) Schematic illustrations of expected effects of oligomycin (ATPase inhibitor), FCCP (mobile ion carrier), 2-deoxy-D-glucose (2-DG, glucoprivic agent), and antimycin A (cytochrome C reductase blocker) and rotenone (complex I blocker) on oxygen consumption rate (OCR) (A) and extracellular acidification rate (ECAR) (B). (C–H) Comparative OCR or ECAR analysis of isolated Siglec-7+ and Siglec-7- CD8+ T cells from healthy donors by Seahorse (three independent experiments with 2-4 donors each, 11 donors in total). OCR profile (C), basal respiration (steady state mitochondrial respiration) (D), and ATP-linked respiration (ΔOCR after oligomycin injection) (E). ECAR profile (F), basal acidification (ECAR at steady state) (G), and glycolysis (ΔECAR at steady state minus non-glycolytic acidification in response to 2-DG injection) (H). Statistical analysis was performed by paired t test (D, E, G, H). *P < 0.05; n.s., not significant. Error bars, SD (D, E, G, H) or SEM (C, F).