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. Author manuscript; available in PMC: 2022 Nov 16.
Published in final edited form as: Nat Catal. 2022 May 16;5(5):443–454. doi: 10.1038/s41929-022-00782-7

Fig. 3. Requirement of sulphite-type species for substrate turnover.

Fig. 3.

(a) Activities of Av1* in in vitro activity assays containing Av2, MgATP and Eu(II)-EGTA in the absence −S) or presence of various sulphur sources (+S2−, +SO42− and +SO32−) or selenite (+SeO32−). (b) Titration of the C2H2-reducing activity of Av1* versus increasing concentrations of sulphite (SO32−) or selenite (SeO32−) in in vitro assays containing Av2, MgATP and Eu(II)-EGTA. (c) Time-dependence of the activity of Av1* in C2H2 reduction using 2 mM SO32− (green solid circles) or nitrogenase-free extract (designated NFE; open circles) as a sulphur source. 318±36 and 250±16 nmol C2H4/mg were generated in assays containing SO32− and NFE, respectively. Data in a-c are expressed as mean ± s.d. (n=3 independent experiments).