TABLE 1.
N-terminal amino acid sequences of 2-aminobenzoate-CoA ligase and ACMR, as determined by Edman degradationa
| Enzyme (method) | Amino acid sequenceb |
|---|---|
| ACMR (Edman degradation) | MRIVc IGGGP AGLYF AILMK KLNP(V/A) HEIrV IerNr (A/P)YDTF G |
| ACMR (deduced from ORF 1, type I) | MRIVC IGGGP AGLYF AILMK KLNPV HEIR V VERNR AYDTF G |
| ACMR (deduced from ORF 1, type II) | MRIVC IGGGP AGLYF AILMK KLNPA HEIRV IERNR PYDTF G |
| 2-AB-CoA ligasec (Edman degradation) | TSHVD TFARD XLPPX eQQ |
| 2-AB-CoA ligase (deduced from ORF 7, type I) | TSHVD TFARD RLPPP ELQ |
| 2-AB-CoA ligase (deduced from ORF 7, type II) | TSHVD TFARD RLPPP EQQ |
a
Deduced amino acid sequences of ORFs 1 and 7 (ACMR and 2-aminobenzoate-CoA ligase, respectively), which exist as duplicate copies I and II with minor differences (boldfaced amino acids), are included.
b
Lowercase letters, residues of uncertain identity; X, unknown amino acid; V/A and A/P, mixtures of V and A and of A and P, respectively.
c
2-AB-CoA ligase, 2-aminobenzoate-CoA ligase.