Skip to main content
. 2022 Aug 17;73(3):e12821. doi: 10.1111/jpi.12821

Figure 5.

Figure 5

Developmental hypoxia does not change oxidative capacity in heart mitochondria from female fetuses. Mitochondrial oxidative capacity was measured following a SUIT (substrate‐uncoupler‐inhibitor‐titration) protocol. Each panel represents a different respiratory state. RCR values (G) and coupling efficiencies (H) were high for all groups, demonstrating mitochondrial homogenate preparations were of good quality. n = 9 (NC from 7 litters), 7 (NM from 5 litters), 11 (HC from 8 litters), 9 (HM from 7 litters). Significance was assessed using a linear mixed model (nested). Error bars show mean ± SEM. 1‐(L/P), coupling efficiency; CI, complex I; CII, complex II; CIV, complex IV; ETC, electron transport capacity; HC, hypoxia control; HM, hypoxia melatonin; NC, normoxia control; NM, normoxia melatonin; OXPHOS, oxidative phosphorylation; RCR, respiratory control ratio; SUIT, substrate–uncoupler–inhibitor–titration.