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. 2022 Aug 17;73(3):e12821. doi: 10.1111/jpi.12821

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© 2022 The Authors. Journal of Pineal Research published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effects of developmental hypoxia on citrate synthase activity and lipid peroxidation in fetal ventricles. Citrate synthase activity and MDA were measured in homogenized ventricular tissue from male (A, B) and female (C, D) fetuses. Circular symbols show males, triangular symbols show females. For MDA (males), n = 8 (NC from 6 litters), 6 (NM from 6 litters), 5 (HC from 4 litters), 7 (HM from 6 litters). For MDA (females), n = 3 (NC from 3 litters), 6 (NM from 5 litters), 7 (HC from 6 litters), 7 (HM from 6 litters). For citrate synthase (males), n = 10 (NC from 7 litters), 14 (NM from 7 litters), 11 (HC from 6 litters), 9 (HM from 6 litters). For citrate synthase (females), n = 8 (NC from 6 litters), 3 (NM from 3 litters), 8 (HC from 6 litters), 9 (HM from 7 litters). Significance was assessed using a linear mixed model (nested). Error bars show mean ± SEM. HC, hypoxia control; HM, hypoxia melatonin; MDA, malondialdehyde; NC, normoxia control; NM, normoxia melatonin; OD, optical density.