TABLE 2.
Serotype, sequence type and number of isolates harbouring virulence genes among the 31 STEC strains from red deer analyzed by whole genome sequencing
| O146:H28 ST738 (n = 10) | O113:H4 ST10 (n = 4) | O187:H28 ST200 (n = 4) | O91:H14 ST33 (n = 3) | O91:H21 ST442 (n = 1) | O27:H30 ST53 (n = 3) | O104:H7 ST1817 (n = 1) | O174:H8 ST13 (n = 1) | O178:H19 ST443 (n = 1) | ONT:H49 ST5418 (n = 3) | Total (%) | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Shiga‐toxin pattern † | stx1a | 2 | 3 | 5 (16.1) | ||||||||
| stx1c | 2 | 1 | 3 (9.7) | |||||||||
| stx2b | 10 | 1 | 3 | 1 | 15 (48.4) | |||||||
| stx2g | 4 | 4 (12.9) | ||||||||||
| stx1a+stx2a | 1 | 1 (3.2) | ||||||||||
| stx1a+stx2b | 1 | 1 (3.2) | ||||||||||
| stx1c+stx2b | 2 | 2 (6.5) | ||||||||||
| Genes involved in colonisation ‡ | eae | 0 | ||||||||||
| iha | 10 | 4 | 3 | 1 | 3 | 1 | 1 | 3 | 26 (83.9) | |||
| saa | 3 | 1 | 4 (12.9) | |||||||||
| lpfA | 10 | 4 | 3 | 1 | 1 | 1 | 1 | 3 | 24 (77) | |||
| tia | 4 | 3 | 1 | 3 | 1 | 1 | 13 (41.9) | |||||
| aggR | 0 | |||||||||||
| aaiC | 1 | 3 | 4 (12.9) | |||||||||
| aat | 0 | |||||||||||
| Genes encoding toxins § | ehxA A | 4 | 3 | 1 | 1 | 1 | 10 (32.3) | |||||
| ehxA D | 4 | 4 (12.9) | ||||||||||
| astA | 10 | 4 | 4 | 1 | 3 | 22 (71) | ||||||
| subAB | 10 | 4 | 3 | 1 | 3 | 21 (67.7) | ||||||
| sta1 | 4 | 4 (12.9) | ||||||||||
| ltcA | 0 |
stx, Shiga toxin subtype.
eae, intimin; iha, encoding the adherence‐conferring protein Iha, a homologue of Vibrio cholera IrgAiron‐regulated gene A homolog adhesion similar to V. cholerae; saa, STEC agglutinating adhesin; lpfA, long polar fimbriae closely related to LPF of Salmonella enterica serovar Typhimuriuman; tia, invasion determinant of the subtilase‐encoding pathogenicity island of LEE‐negative STEC; aggR, transcriptional activator of aggregative adherence fimbria I expression of EAEC; aaiC, EAEC aggR‐activated island C; aat, EAEC‐associated anti‐aggregation transporter.
ehxA A, enterohemolysin‐encoding gene subtype A; ehxA D, enterohemolysin‐encoding gene subtype D; astA, EAEC heat‐stable toxin; subAB, subtilase cytotoxin locus; sta1, ETEC heat‐stable enterotoxin 1; ltcA, ETEC heat‐labile enterotoxin.