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. 2022 May 25;70(9):1762–1776. doi: 10.1002/glia.24218

FIGURE 4.

FIGURE 4

The expression of Tgfβ receptors was suppressed, and Igf1 upregulated, in hypoxic microglia of oxygen‐induced retinopathy (OIR) mice. (a) Schematic showing the protocol used to generate data for the OIR experiment in this figure. (b) The hypoxic retina in P15 OIR mice was detected by Hypoxyprobe‐1™ (green) (scale bars = 1 mm). (c) The representative flow‐sorting scatter stained by Gr‐1 and Cd11b. (d) The expression of Tgfβb1, Tgfβbr1, Tgfβbr2, Igf1, and Vegfa in Cd11b+ and Gr‐1‐ microglia sorted out from P15 OIR and normoxic mice was determined by qPCR (n = 7 each). Data are mean ± SEM. p Values were calculated using a two‐tailed Student's t‐test, *p < .05, **p < .01. N.S., not significant. (e) The expression of Tgfβb1, Tgfβbr1, Tgfβbr2, Igf1, and Vegfa in whole retina from P15 OIR and normoxic mice was determined by qPCR (n = 4 each). Data are mean ± SEM. p Values were calculated using a two‐tailed Student's t‐test, ***p < .001. N.S., not significant.