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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Nat Aging. 2022 May 16;2(6):494–507. doi: 10.1038/s43587-022-00214-y

Extended Data Figure 8. Related to Figure 3. Ubiquitous, gut-, or muscle-specific BNIP3 induction shortens lifespan.

Extended Data Figure 8.

(a) Survival curves of daGS>UAS-BNIP3 flies with or without RU486-mediated transgene induction from day 5 onward. ***p=0.0006 (RU0 vs. RU5) and ***p<0.0001 (RU0 vs. each other RU dose), (n.s.) non-significant; log-rank test; n = 208 RU−, 200 RU5, 204 RU10, 198 RU25, and 201 RU50 biologically independent animals.

(b) Survival curves of 5966GS>UAS-BNIP3 flies with or without RU486-mediated transgene induction from day 5 onward. ***p<0.0001 (RU0 vs. each RU dose); log-rank test; n = 201 RU−, 194 RU5, 197 RU10, 199 RU25, and 204 RU50 biologically independent animals.

(c) Survival curves of Act88FGS>UAS-BNIP3 flies with or without RU486-mediated transgene induction from day 5 onward. ***p<0.0001 (RU0 vs. each RU dose); log-rank test; n = 239 RU−, 473 RU5, 233 RU10, 234 RU25, and 238 RU50 biologically independent animals.

(d) Survival curves of daGS>UAS-BNIP3-RNAi flies with or without RU486-mediated transgene induction from day 5 onward. ***p=0.0006; log-rank test; n = 209 RU− and 233 RU+ biologically independent animals.

(e) Survival curves of daGS>UAS-BNIP3-RNAi flies with or without RU486-mediated transgene induction from day 30 onward. ***p<0.001; log-rank test; n = 209 RU− and 202 RU+ biologically independent animals.

(f) Survival curves of elavGS>UAS-BNIP3-RNAi flies with or without RU486-mediated transgene induction from day 5 onward. *p=0.0203; log-rank test; n = 165 RU− and 180 RU+ biologically independent animals.