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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Nat Aging. 2022 May 16;2(6):494–507. doi: 10.1038/s43587-022-00214-y

Extended Data Figure 1. Related to Figure 1. RU486 induces BNIP3 expression in the brain of elavGS>UAS-BNIP3 flies.

Extended Data Figure 1.

(a) Immunostaining of brains from 10-day-old elavGS>UAS-BNIP3 flies with or without RU486-mediated transgene induction from day 5 onward, showing BNIP3 expression level (green channel, anti-HA) and nuclear DNA (blue channel, stained with DAPI). Scale bar is 50 μm.

(b) Quantification of BNIP3 expression level in brain as shown in (a). n = 10 biologically independent animals per condition. ***p<0.0001; unpaired t test. Data are presented as scatter plots overlaying mean values +/− SEM.

(c) Western blot detection of BNIP3 transgene induction in the heads of day 14 elavGS>UAS-BNIP3 flies with or without 9 days of RU486 treatment. n = 5 biological replicates per condition with 10 flies pooled per replicate.

(d) Immunostaining of brains from young (10-day-old) elavGS>UAS-BNIP3 flies with or without RU486-mediated transgene induction from day 5 onward, showing mitochondrial morphology (green channel, anti-ATP5a) and BNIP3 (red channel). Arrows and outlines indicate sites of colocalization. Scale bar is 3 μm. RU486 was provided in the media at a concentration of 5 μg/ml. Images are representative of 5 samples treated with RU and 6 samples provided vehicle.