Fig. 5.

Cirsiliol binds with STAT3 and inhibits STAT3 nuclear translocation. A Chemical structure of cirsiliol. B Image of molecular docking of cirsiliol and STAT3 (PDB ID: 1BG1). C Schematic of the interaction of cirsiliol and STAT3. D–F HCT116 cells were exposed to cirsiliol for 24 h. Representative western blot images (D) and quantitative analysis (E) for p-STAT3 and STAT3. Representative immunofluorescence images of STAT3 (F). G–I For IL-6-treated group, HCT116 cells were stimulated by IL-6 (25 ng/ml, 30 min). For cirsiliol treatment, HCT116 cells were treated with cirsiliol (20 μM) for 24 h and stimulated with IL-6 (25 ng/ml) for another 30 min. Representative images of western blot (G) and quantitative analysis (H) for p-STAT3 and STAT3. Representative immunofluorescence images of STAT3 (I). n = 3 (d-e, g-h) per group. Data represent means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 vs ctrl. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs IL-6 stimulation