Fig. 6.

Effect of estrogen receptor antagonist ICI182,780 on JAK2/STAT3 pathway inhibited by calycosin in TGF-β1-induced LX-2 cells. A Representative blots of p-JAK2, JAK2, p-STAT3, STAT3 proteins detected by western blot. B, C Relative quantification analysis of p-JAK2, JAK2, p-STAT3, STAT3 protein expressions and the ratios of p-JAK2/ JAK2 and p-STAT3/STAT3. GAPDH was used as an internal control. Data are shown as mean ± SD of three independent experiments. ^ΔΔp < 0.01, ^ΔΔΔp < 0.001 vs control group; **p < 0.01 vs TGF-β1 group; ⁺p < 0.05, ⁺⁺p < 0.01 vs the corresponding treatment groups without ERβ overexpression