FIG. 4.
Transient destabilization of RpoH during the heat shock response. A log-phase culture of wild-type cells (GV3101) grown in synthetic medium at 25°C was divided into two portions, pulse-labeled with [35S]methionine for 2 min, and chased with excess unlabeled methionine. Samples were taken after 3 min and then at 10-min intervals. At the time of second sampling (time zero), one culture was shifted to 37°C (●), whereas the other was kept at 25°C (▪). Immunoprecipitates obtained with anti-RpoH antiserum were analyzed by SDS-PAGE. Averages from three experiments are plotted with standard errors (indicated by the error bars).