TABLE 2.
Autogenous control of RpoH-dependent transcription of rpoHa
| Strain and plasmid | β-Galactosidase activity |
|---|---|
| KN613 (rpoH+) | |
| pUCD-lacZ | 0.6 ± 0.4 |
| pUCD-PrpoH′-lacZ | 1,011 ± 14.4 |
| KN201 (ΔrpoH) | |
| pUCD-lacZ | 1.4 ± 0.5 |
| pUCD-PrpoH′-lacZ | 41.4 ± 0.4 |
a
Plasmid carrying the promoterless lacZ or lacZ driven by the authentic PrpoH promoter was inserted in the rpoH+ or ΔrpoH strain as indicated, and the resulting transformants were grown in synthetic medium at 25°C to determine β-galactosidase activity. Averages of at least three independent measurements with standard errors are presented in Miller units.